Investigation of mutations in Exon 7,8 and Exon 9 of FHIT Gene in laryngeal squamous cell carcinoma

The purpose of this study was to assess and explain the relationship between molecular changes occurring in squamous cell laryngeal carcinoma. Furthermore, we were revealed the role of FHIT gene response in the development and progression of squamous cell laryngeal carcinoma, and genes based exons 7,8 and 9 in tumour stage and histopathologic grade. In this study was used 50 blood samples for control and 26 patients with laryngeal squamous cell carcinoma. Briefly, for this purpose, we primarily aimed researching or evaluating exon 7 (C/T), exon 8 (T/C) and exon 9 (A/G) homozygous deletions of FHIT gene exons. Mutations were examined with polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and RT-PCR based HRM technigues. At the end of the HRM analysis, was observed a significant mutation in the groups of patients. L4 and L19 in the number and before the results of SSCP individuals identified as benign according to the results of HRM. In this study, exon 7 and 9 healthy individuals and squamous cell laryngeal carcinoma were observed any difference between individuals in terms of mutation. The pathology of the case was low differentiation. But in addition to this findings, the present findings highlight that the FHIT gene partially related to target for analysis in case with laryngeal squamous cell carcinoma. According to results of analyses, we idendified a link in between the exon 8 and exon 9 of FHIT gene. Abbreviations: SCC: squamous cell carcinoma; PCR-SSCP: polymerase chain reaction single-strand conformation polymorphism; HRM: high-resolution melting